دی‌اتیل‌پیروکربنات سیگما

Diethyl pyrocarbonat

D5758

صفحه کالا در سایت سیگما(کلیک کنید)

برگه انالیز کالا (کلیک کنید)

Back

D5758

All Photos(3)

Key Documents

• SDS

• COO/COA

• Specification Sheet

View All Documentation

D5758

Share

Diethyl pyrocarbonate

5.0 out of 5 stars, average rating value. Read 2 Reviews. Same page link.

5.0

(2)

1 of 2 reviewers received a sample product or took part in a promotion

Write a review

Ask a question

96% (NT)

Synonym(s):

DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride

Sign Into View Organizational & Contract Pricing

Select a Size

Change View

5 ML

€64.20

25 ML

€191.00

50 ML

€324.00

100 ML

€583.00

About This Item

Linear Formula:

O(COOC2H5)2

CAS Number:

1609-47-8

Molecular Weight:

162.14

Beilstein:

637031

EC Number:

216-542-8

MDL number:

MFCD00009106

UNSPSC Code:

12352202

PubChem Substance ID:

24893964

NACRES:

NA.77

D5758-5ML

€64.20

Availability

Available to ship onDecember 24, 2024Details

To order products, please contact your local dealer.

Properties

biological source

synthetic (microbial fermentation/ cell culture)

Quality Level

200

Assay

96% (NT)

form

liquid

refractive index

n20/D 1.398 (lit.)

bp

93-94 °C/18 mmHg (lit.)

density

1.101 g/mL at 25 °C (lit.)

shipped in

wet ice

storage temp.

2-8°C

SMILES string

CCOC(=O)OC(=O)OCC

InChI

1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3

InChI key

FFYPMLJYZAEMQB-UHFFFAOYSA-N

Show Less

Description

General description

Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.

Application

Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases.[1] It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.[2]

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Biochem/physiol Actions

DEPC is a potent nuclease inhibitor.[3][4]0.1% solution of DEPC is used to inactive RNase.
It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose and time dependent manner and increases the Ca2+ permeability of SR vesicles.[5]

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

Safety Information

Pictograms

GHS07

Signal Word

Warning

Hazard Statements

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

156.2 °F - closed cup

Flash Point(C)

69 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves